Abstract: To explore the possible mechanism of Huayu Tongluo traditional Chinese medicine in reducing proteinuria and protecting renal function of diabetic nephropathy on expression of podocin,CD2AP in the conditionally immortalized mouse podocytes.The conditionally immortalized mouse podocytes were selected as the research object.These podocytes cultured under the growth permissive condition were divided into the normal glucose group (CG group),high glucose group (HG group) and high glucose dosing group (HG+Z group) and intervened for 24 h,48 h and 72 hours.The expression of podocin and CD2AP was detected by ICC,Western-blot and Real-Time PCR.It was observed that at 48 hours,compared with CG group,the expression of podocin and CD2AP protein in HG and (HG+Z) groups was lower (P<0.05,P<0.01).Compared with HG group,the expression of podocin and CD2AP protein in (HG+Z) group was higher (P<0.05).Compared with CG group,at same time point,the expression of podocin and CD2AP mRNA in other groups was lower (P<0.01).Compared with HG group,only at 48 hours,the expression of podocin and CD2AP mRNA in (HG+Z) group was higher (P<0.01).The results indicated that Huayu Tongluo traditional Chinese medicine can increase the expression of membrane protein podocin,CD2AP and it may be one of the mechanisms on reducing diabetic nephropathy proteinuria and protecting renal function.

Key words: Huayu Tongluo Chinese herbs, slit diaphragm protein, podocin, CD2AP