Abstract: High-voltage pulsed electric field (PEF) is an emerging non-thermal processing technology that has recently generated much interest for improving the extraction rate of some bioactive substances and enhancing the quality of food products.To improve the extraction rate of phosphatidylcholine (PC) from sika deer velvet antler slag,PEF-assisted organic solvent was applied in this study.The quantitative effects of field strength,pulse numbers,moisture content of extraction solvent,and a three-level Box-Behnken design which was based on the results of a single-factor test was employed to optimize the parameters for extraction of PC.By solving the regression equation and also by analyzing the response surface contour plots,the optimal PC extraction conditions were determined:field strength 20 kV/cm,pulse numbers 9 and moisture content of extraction solvent 10.5,and the weight of PC extracted was 1.592 mg per 1g sika deer velvet antler slag.

Key words: high-voltage pulsed electric field, phosphatidylcholine, Box-Behnken