Abstract: In order to understand the effects of location on the chemical composition and anti-inflammatory effects of Maqian(Zanthoxylummyriacanthum var.pubescens Huang) essential oil(MQEO),fruits of Maqian were collected from three locations in Xishuangbanna(Menglun,Mengwang,Mengxing).The essential oils were obtained by hydrodistillation.The chemical composition was analyzed using GC-MS and the anti-inflammatory effect was determined by RAW264.7 cells.A total of 21 kinds of substances were identified in three MQEOs among which alkenes and alcohols were the dominant components.The chemical composition of MQEOs from Menglun and Mengxing was more similar,whereas MQEO from Mengwang showed marked difference in the content of several major components from those in Menglun and Mengwang samples.RAW264.7 cells were pretreated with vehicle,Dex,or MQEO,and then cultured with LPS for 24 h.NO was determined by Griess reagent.TNF-α was determined by ELISA.Three MQEO all exhibited dose-dependent inhibition of LPS-stimulated production of NO and TNF-α.These results showed that despite difference in the chemical composition of MQEO from three locations,their anti-inflammatory effects remain the same.

Key words: Maqian essential oil, producing area, chemical composition, RAW264.7, anti-inflammatory