Abstract: In order to investigate bioactive constituents with inhibiting effect on benign prostatic hyperplasia from Oiltea camellia, preparative high-performance liquid chromatography(preparative HPLC) was adopted to isolate key bioactive compounds from polyamide fraction of O.camellia extracts(OCE).The chemical structures of those compounds were identified using mass spectrometer(MS) and nuclear magnetic resonance(NMR) analyses.In addition,its growth inhibitory activity against BPH-1 cells was detected by MTT assay.The results indicated that F1,F2 and F3,as main active substances in Fr8,showed stronger inhibitory effect on BPH-1 cells at low doses(50 μg/mL) than that of positive control finasteride(26.50%),with the inhibition rates of 30.31%,43.26% and 29.00%,respectively.F1,F2 and F3 were identified as valoneic acid dilactone,3-O-galloyl-4,6-［(S)-hexahydroxydiphenoyl］-(α/β)-D-glucopyranose and 3,4,6tri-O-galloyl-(α/β)-D-glucose,respectively.This study provided theoretical reference and material foundation for the development of natural products on the improvement of prostate disease.

Key words: Oiltea camellia, polyamide fraction, preparative high-performance liquid chromatography, structural identification, BPH-1 cell