Abstract: Near-infrared spectroscopy method was established for the rapid determination of luteolin in Chrysanthemum indicum made by different processing methods and from different producing areas of Henan.Three manufacturing methods(drying directly,drying after steaming,drying after firing) were adopted to process wild chrysanthemum flower from Xinzheng,Sanmenxia and Luoyang,respectively.The content of luteolin in the C.indicum was measured by HPLC.The NIRS spectra of wild Chrysanthemum were collected.Then,the near-infrared spectroscopy was combined with PLS analysis to establish the quantitative model of luteolin in C.indicum. The correlation coefficients(R²),root mean square error of calibration(RMSEC) and the root mean square error of predication(RMSEP) of the established quantitative analysis model of luteolin were 0.94525,0.0142 and 0.0428,respectively.The near infrared prediction correlation coefficient of luteolin content in validation sets samples was 2.95%,which suggested good quality of prediction.The method was efficient,rapid and accurate,and can be used for the rapid determination of the content of luteolin in C.indicum from Henan.

Key words: Chrysanthemum indicum, different manufacturing methods, luteolin, NIRS, rapid determination