Abstract: To explore the underlying protection and mechanism of RSV in renal ischemia reperfusion injury(IRI). The model of IRI was induced by clamping the left renal pedicles with right nephrectomy. SD rats were randomly divided into Sham group(Sham),Renal ischemia reperfusion injury group(IRI),and Resveratrol pretreatment group(RSV). The morphology of kidney was exanimed by HE;The expression of Cr and BUN were measured by ELISA;TUNEL was used to examine the cell apoptosis;The expression of SIRT1,p53,Acetylp53,Bax,BCL-2,PUMA-α and cytochrome c were evaluated by Western blot analysis. The results showed RSV pretreatment can significantly decrease the expression of TUNEL,BUN,Cr,Acetyl 53,PUMA-α and Bax by increaseing the expression of SIRT1and BCL-2. Moreover,RSV pretreatment can also attenuate the change of morphology of kidney and the transcolation of cytochrome c,which indicated RSV pretreatment can protect kidney against ischemia reperfusion injury by suppressing apoptosis. The mechanism was associated with attenuating Acetylp53 by activating SIRT1.

Key words: resveratrol, renal ischemia reperfusion injury, apoptosis, SIRT1/p53