Abstract: To optimize the separation and purification technology of salvianolic acid A from Salvia miltiorrhiza var. alba with polymide resin.The antioxidant activity of the purified salvianolic acid A were studied in vitro. With adsorption capacity and desorption rate as index,static and dynamic adsorption-desorption were used to determine the optimal separation and purification conditions of salvianolic acid A.The optimal conditions of polymide resin were as follows:sample injection concentration was 11 g/L,sample flow rate was 1.0 mL/min,quantity of adsorption was 150 mL,desorption rate was 1.0 mL/min,and elution volume was 10 BV of 50% ethanol.After the purification,the purity of obtained salvianolic acid A was 40.36%.Moreover,the antioxidant activity of the extract were evaluated by DPPH scavenging assay and reducing power.The results showed that the obtained salvianolic acid A had good antioxidant activity.

Key words: Salvia miltiorrhiza var.alba, salvianolic acid A, polymide resin, optimal process, antioxidant activity