Abstract: In this paper,the GCMS and computer retrieval technique were applied to analyze and identify the ethereal extracts of oil and aqueous phase which were obtained from Nasturtium officinale R.Br derived from Kunming,Yunnan province,by steam distillation method.The results showed that various ingredients were detected in the extract,such as terpenoids,alcohols,aldehydes,acids,ethers and so on,among which the isothiocyanates were richest.The compounds of 26 were identified,88.227% of the total peak areas were identified.its content of isothiocyanates respectively 4Methylpentyl isothiocyanate (0.019%),1isothiocyanatobutane (0.111%),1isothiocyanatoheptane (0.014%),2isothiocyanatoethylbenzene (85.282%),nonyl isothiocyanates (0.021%),3isothiocyanatopropylbenzene (0.042%),accounting for 85.489%.The constituents of Nonvolatile which 44 peaks were separated,24 compounds were identified and accounted for over 83.743%,which contains 2isothiocyanatoethylbenzene accounted for 43.539%,Benzenepropanenitrile (15.933%),2methoxy4vinyl phenol (10.578%),diphenylamine (6.368%) etc.Furthermore,systematic analysis was conducted on the compositions and their relative contents of the volatile and nonvolatile components in N.officinale. which could build foundation for further utilization of this species.

Key words: Nasturtium officinale R.Br, isothiocyanates , chemical constituents, GCMS