Abstract: To investigate the effects of crude polysaccharide from Arenaria kansuensis (AKCP) on the proliferation and function of murine immunocyte in vitro. After mice peritoneal macrophages and spleen lymphocytes were cultured in vitro and be treated by different concentrations of AKCP for 48 hours,the lymphocyte proliferation and NK killing activity were detected by MTT method,the CD3⁺/CD4⁺/CD8⁺ subsets in spleen lymphocytes were analyzed by FCM,and the levels of IL-2 and IFN-γ in spleen cell culture supernatant were tested by ELISA.The results showed that after high,middle and low concentrations AKCP treatment,the phagocytic and NO secreting activities of peritoneal macrophages,the proliferation index as well as IFN-γ secretion level of lymphocytes,and the NK killing activity were significantly higher than those in control group (P<0.05).In middle AKCP group,the CD3⁺,CD4⁺ subset in spleen lymphocytes,the level of IL-2 secreted by lymphocytes was obviously increased (P<0.05).These results collectively indicated that AKCP had abilities to activate the proliferation and function of murine immunocyte in vitro.

Key words: Arenaria kansuensis, polysaccharide, immune, activation, in vitro