Abstract: In order to evaluate the whitening activities of secondary metabolic products from endophytic fungi derived from Fritillaria cirrhosa, F.unibracteata var. wabuensis, F.przewalskii and F.unibracteata, the inhibitory activity of the fungi against tyrosinase were measured.In addition,the crude extracts of 3 fungi screened from 15 endophytic fungi according to inhibitory activities were obtained using petroleum ether (30-60 ℃),ethyl acetate and n-butanol.Vc was used as the positive control.The results showed that the inhibition abilities of strain WBS020,1WBY2 and 7WBY2 against tyrosinase were significantly higher thanother stains,and maintained stable after heat treatment.The ethyl acetate and n-butanol extracts of WBS020,1WBY2 and 7WBY2 displayed a significantly higher inhibition activity against tyrosinase than the petroleum ether.And the n-butanol fraction from strain WBS020 showed the strongest activity,while ethyl acetate fractions from 1WBY2 and 7WBY2 showed the strongest activities.In addition,the inhibition rates of Vc was significantly higher than these of other extracts,but the ethyl acetate extracts from strain 1WBY2 and 7WBY2 still reach to 90.86%± 0.81% and 88.92%±7.13% at 8 mg/mL,respectively.The results showed that there were whitening active ingredients in secondary metabolites of endophytic fungi from Fritillaria unibracteata var.wabuensis,which had potential exploitation and utilization values.

Key words: Fritillariae Cirrhosae Bulbus, endophytic fungi, tyrosinase, whitening activates