Abstract: A method for simultaneous extraction of tanshinone IIA and salvianolic acid B from the Salvia miltiorrhiza Bunge and the purification of salvianolic acid B was developed.The extraction process was optimized by orthogonal design with the content of tanshinone IIA and slvianolic acid B as evaluation indexes.The optimal extraction process was as follows: S.miltiorrhiza powder was extracted by 8 times of 70% ethanol with at 85 ℃ (reflux) for three times in 1 h.The extraction rate of tanshinone IIA was higher than 90%,and the extraction rate of salvianolic acid B was higher than 80%.The extract was dissolved in hot waterand filtered,the filtrate was concentrated to 1 mL of a solution containing about 0.5 g of crude drugwith the volume of 100 mL was loaded on the D101macroporous resin column with the sample flow rate of 2 BV/h,and then column was washed by 6 BV of water,8 BV of 30% ethanol with the flow rate of 4 BV/h for removing impurities,10 BV of 60% ethanol with 2 BV/h flow rate for purifying,to obtain more than 75% purity of salvianolic acid B,verification experiments showed that the process was stable and feasible;Finally,after washing and methanol recrystallization,more than 99% purity salvianolic acid B was obtained,the total yield of about 2.86% (based onthe dried rhizomes).

Key words: tanshinoneIIA, salvianolicacid B, extraction, macroporousresin, purification