Abstract: To observe the protective effect of different combinations of Chinese recipe of stasis-removing-and-collaterals-dredging on the kidney of diabetic nephropathy (DN) rats and its effect on the expression of slit diaphragm protein P-cadherin in the podocyte,70 male Sprague Dawleg rats were randomly divided into control group,model group,stasis-removing-and-collaterals-dredging group,irbesartan group,stasis-removing group and collaterals-dredging group,and different medicines were intragastrically fed once a day for 16 weeks.Body weight,kidney weight,kidney index and 24 h urinary total protein were tested at the end of week 16.The expression of P-cadherin was detected by Real-time PCR,Western blot,respectively.The results showed that compared with the model group,kidney index,24 h urinary total protein of stasis-removing-and-collaterals-dredging group were significantly decreased (P<0.01),body weight and the mRNA and protein expression of P-cadherin were significantly increased (P<0.01).It suggested that Chinese medicines of stasis-removing-and-collaterals-dredging may increase the mRNA and protein expression of P-cadherin to reduce urinary protein and delay the progression of DN.

Key words: diabetic nephropathy, stasis removing and collaterals dredging, P-cadherin, slit diaphragm protein