Optimization of Deproteinization Process of Exopolysaccharides from Rhizopusnigricans by Response Surface Methodology

doi:10.16333/j.1001-6880.2017.10.023

Abstract

Abstract: The effects of papain concentration,enzymolysis temperature and time on the deproteinization of extracellular polysaccharide from Rhizopusnigricans in Enzyme-Sevag process were investigated by single-factor experiment combining with central composite design method of Box-Behnken.The optimal process parameters were determined based on the deproteinization rate and comprehensive index.After optimizing by response surface methodology,the optimal process parameters were confirmed as follows:papain concentration of 425 U/mL,enzymolysis temperature of 62 ℃ and time of 90 min.Following repeated experiments three times,the average deproteinization rateand comprehensive index were determined as 54.08 % and 2.07,respectively.

Key words: Rhizopusnigricans, exopolysaccharides, deproteinization, enzyme-sevag process, response surface methodology

CLC Number:

R284.2

QIN Guo-zheng1,2,3, JU Yong1, ZHANG Ming-rui1, LI Ping1,2,3, LIU Chun-yan2,3, TANG Bao-lu1, XUE Xin-lei1, WANG Guo-dong1,2,3*. Optimization of Deproteinization Process of Exopolysaccharides from Rhizopusnigricans by Response Surface Methodology[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2017, 29(10): 1773-1778.

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Optimization of Deproteinization Process of Exopolysaccharides from Rhizopusnigricans by Response Surface Methodology

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