NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2017, Vol. 29 ›› Issue (11): 1813-1817.doi: 10.16333/j.1001-6880.2017.11.001

• Article •     Next Articles

Immune Regulation Influence of Melittin on T Cells Induced Differentiation by Mature Dendritic Cells

XIONG Lang-ping1,HE Shou-di2,GUAN Tong3,MO Li-hua2,YANG Ping-chang2,LIU Zhi-gang2,JI Qiong-mei2,HUANG Sheng-guang2*   

  1. 1Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China; 2Institute of Allergy and Immunology, Shenzhen University School of Medicine, Shenzhen 518060, China; 3The NO.1 Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China
  • Online:2017-11-29 Published:2017-11-30
  • Supported by:

    国家自然科学基金（81271950）；深圳市科技计划（JCYJ20130402151227168；JCYJ20150402152130192；005177）；深圳市南山区科技项目（2014046）

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Abstract: To study the intervention of melittin (MT) on dendritic cells (DC) co-cultured with lymphocytes stimulated by lipopolysaccharide (LPS),the impact of MT on the differentiation of Th1/Th2 was investigated.MTS was used to detect the activity of DC co-cultured with lymphocytes by LPS and MT;Flow cytometry was used to detect the ratio of Th1 and Th2 in the co-culture of DCs stimulated by MT and LPS;ELISA was used to detect the concentration IL-4,INF-γ in cell supernatant of MT and LPS stimulated co-cultured DC and lymphocyte.The results showed that a certain concentration of MT and LPS can stimulate the co-cultured activity of DC with lymphocytes.In the induction of mature DC,MT can increase the Th1/Th2 ratio,the Th1 percentage (P<0.05) and the IFN-γ (P<0.01).But it had no effect on the concentration of IL-4 and Th2 (P>0.05).The study demonstrated that through upregulating the expression of Th1 cell ratio and IFN-γ concentration,MT promoted the differentiation of Th1/Th2 cells to the Th1 pathway,thus playing immunomodulatory effects on T cells.

Key words: melittin, dendritic cell, T cell, immune regulation

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