Abstract: The present study described the protective mechanism of purslane on acute eczema induced by DNCB in rats. Rats acute eczema model were established by DNCB,then the eczema rats were treated with purslane extract (20,10,5 g/kg) transdermally once a day,for total 10 days. At the end of medication,rats eczema level was evaluated,skin TEWL was measured,levels and distributions of LTA4,SP,HT H1,IL-31,PAR2 and TRPV1 were detected by immunohistochemistry method,and Filaggrin gene and protein were detected by RT-PCR and Western-blot. The results showed that purslane can significantly reduce rats eczema scores,rats TEWL and the expression of LTA4,SP,HT H1,IL-31,PAR2 and TRPV1 proteins. In addition,Filaggrin gene and protein expression had been significantly increased. The experiment results implied that purslane can significantly protect against the lesions of eczema,which may be associated with decreasing expression of inflammatory cytokines and itching factors and regulate Filaggrin expression in skin barrier system.

Key words: purslane, acute eczema, itch, intervention mechanism