Abstract: An UPLC method was established for simultaneous determination of five flavonoids in the stems of Schisandra chinensis,including rutin,hyperoside,quercetin-3-O-glucoside,quercetin-3-O-xyloside and quercetin.This method was then used to determine the contents of flavonoids in the stems of S.chinensis from different germplasm resources.The five compounds were analyzed simultaneously with an ACQUITY UPLC BEH C₁₈（2.1 mm×5.0 mm，1.7 μm）column by gradient elution using 0.1‰ (v/v) phosphoric acid-acetonitrile as the mobile phase at the flow rate of 0.35 mL/min;The detection wavelength was set at 360 nm,and the column temperture was maintained at 30 ℃.The five flavonoids ingredients of S.chinensis can be separated in the chromatographic conditions.The recoveries of five components were 96.83%～102.15%,with RSD less than 3%.The contents of flavonoids in the stems of S.chinensis  from 19 different germplasm resources had significant differences;94-9-2、130-9-3 and 99-8-7 were significantly better than other strains,94-9-2 was the best.This method was easy to operate and could provide a reference for the quality control of the stem of S.chinensis,and provided theoretical reference for the collection and breeding of S.chinensis germplasm resources.

Key words: Schisandra chinensis, flavonoid, germplasm resource;UPLC