Abstract: This paper firstly offered an effective method of determining Fructus Cannabis phytosterol by gas chromatography (GC).The contents of phytosterol of 5 samples were compared.The results showed that the extraction method of sample 2 had high yield,simple and low cost.Through this repeatable method,a more accurate result can be got.Then a study on the dose-response relationship of phytosterol to intestinal microlflora was made.The method of Co-incubation with human intestinal microflora and phytosterol extract was used to detect the dose responses of the growth of phytosterol to intestinal microflora.The results were analyzed by SPSS Statistics 19.0.In vitro experiment of human intestinal microflora:compared with black control group,as the concentration increased from 0.08516 mg/mL to 0.3407 mg/mL,the number of E.coli increased obviously (P<0.01).Fructus Cannabis extract can promote the proportion of human intestinal microflora positive bacilli in vitro,and can promote the growth of Bifidobacterium.The results showed that phytosterol in Fructus Cannabis can promote the growth of probiotics.

Key words: Cannabis semen phytosterol, content determination, gas chromatography, human intestinal flora, dose-response relationship