Abstract: The characteristic spectra of total saponins of Schizocapsa plantaginea Hance (SFSP_60%) were established by High Performance Liquid Chromatography with Ultraviolet and Evaporative Light Scattering Detector.Acetonitrile-water mobile phase gradient Elution.The detection wavelength was 203 nm,the column temperature was 30 ℃ and the flow rate was 1 mL/min.Sample volume 10 μL.Reference to the SFSP_60% chromatographic peaks in the ELSD chromatogram.Area normalization method is used to calculate saponinⅠof Schizocapsa plantaginea Hance(SPHSⅠ) accounted for 8428%(The relative percentage),the saponinⅡ of Schizocapsa plantaginea Hance (SPHSⅡ) was 3.02%,the compound 1 was 2.21%,the compound 2 was 6.31%,the compound 3 was 2.73%,Compounds 1-3 may be nucleosomal similar saponins.Take the logarithmic growth phase SMMC-7721 cells,cells in the administration group were added to 0,0.25,0.5,2,4 μg/mL SFSP_60%,SPHS Ⅰ,SPHS Ⅱ and SPHS Ⅰ+Ⅱ (SPHS Ⅰ and SPHS Ⅱ accounted for 96.54%,3.46%) for 24,48,72 h.MTT assay was used to detect the proliferation inhibition rate of SMMC-7721 cells in order to finally calculate its half inhibitory concentration (IC₅₀).The results showed that the inhibitory effect of the administration group on the proliferation of SMMC-7721 cells was in a time-and dose-dependent manner (all P<0.05),and the viability size was SFSP_60% ＜ SPHSⅠ,SPHSⅡ ＜ SPHSⅠ+Ⅱ.It was proved that SPHSⅠand SPHSⅡplay the main antitumor effect in total saponins,and the anti-tumor effect is the result of the interaction of each component.Which provided a reference for the study of the viability of effective components in Schizocapsa plantaginea Hance and a new idea for the rational allocation of effective components.

Key words: Schizocapsa plantaginea Hance, total saponins of Schizocapsa plantaginea Hance, characteristics of Saponins of Schizocapsa plantaginea Hance, SPHSⅠ, SPHSⅡ, tumor cell proliferation