Abstract: To establish the HPLC fingerprint of standard decoction of different processed products of Paeoniae Alba Radix (PAR) in Bozhou.The standard decoction of PAR had 16 common peaks,fired PAR had 14 common peak,and PAR with wine had 13 common peak.The seven different chemical composition (including gallic acid,oxypaeoniflorin,alibiflorin,paeoniflorin,benzoic acid,1,2,3,4,6-pentagalloylglucose,benzoylpaeoniflorin) were measured.The content of alibiflorin and benzoic acid increased in the standard decoction after fired,meanwhile oxypaeoniflorin and paeoniflorin reduced.In the standard decoction of PAR with wine,the content of paeoniflorin increased and the content of benzoic acid decreased.The results were analyzed by cluster analysis (CA),the same PAR and processed products can get together for a class,but the different processed products of PAR were clustered into one category after clustering distance was reduced.The HPLC fingerprint and content determination methods had a good reproducibility and was simple as well as fast.The combination of the two methods can reflect the difference of the changes of different processed products of PAR in Bozhou.

Key words: Paeoniae Alba Radix in Bozhou, processed products, standard decoction, fingerprint, content determination