Abstract: The objective of this study was to investigate the anti-aging effects of glacier water and Thamnolia vermicularis extract (TVE) on human dermal fibroblasts (HDF)and their underlying mechanism.The HDF were treated by glacier water and TVE,and then the cell viability,the expression level of extracellular matrix components and corresponding hydrolytic enzymes were examined.The results revealed that both glacier water and TVE can promote the cell proliferation and energy metabolism level of HDF,as well as enhancing cellular content of hyaluronic acid and expression level of elastin.Besides,TVE also showed an ability to up-regulate the expression of collage I and down-regulate the mRNA expression of elastase.Their efficacy was much more obvious when combining glacier water and TVE together.The results suggested that glacier water and TVE had anti-aging effect on skin through enhancing the cellular activity of HDF and promoting expression of extracellular matrix components as well as inhibiting their degradation.

Key words: glacier water, Thamnolia vermicularis, anti-aging, human dermal fibroblasts, cell viability, extracellular matrix