Abstract: To investigate the purification technology of pentacyclic triterpenoids from Paliurus ramosissimus by macroporous resin.The absorption and desorption capacity of 10 resin (DA201,AB-8,D1400,D4020,NKA-9,HPD100,HPD70,H130 and X-5) for betulinic acid and paliurusene were investigated to select the optimal macroporous resin.The adsorption characters and the influencing factors of the selected macroporous resin were also investigated to optimize the purification technology.The optimized conditions were as follows:the concentration of betulinic acid was 16.07 μg/mL,the concentration of paliurusene was 1023 μg/mL,the loading volume was 1000 mL (loading amount was 0.4 g/g),eluent was 85% ethanol,the amount of 900 mL,elution rate of 3 mL/min.After purification of the resin,the content of paliurusene was increased by 8 times,the content of betulinic acid was increased by 5 times,and the content of pigment and other impurities was greatly reduced.The IC₅₀ of Hep-G2 and AGS cells were 30.79±8.49,8.75±2.83 mg/L,respectively,after purification using macroporous resin.The purification process was reasonable,suitable for the purification of active ingredients in Paliurus ramosissimus.

Key words: Paliurus ramosissimus, pentacyclic triterpenes, macroporous resin, purification process