Abstract: Explored the hepatotoxicity effect of eighteen constituents from polygoni multiflori radix with zebrafish model for the first time to provide reference for the hepatotoxicity material basis study of polygoni multiflori radix.The liver fluorescence transgenic zebrafish were given the 18 components of polygoni multiflori radix at high,medium and low doses,respectively for 72 h and these zebrafish were photographed by fluorescence microscope at 24 h,48 h and 72 h after the administration,respectively.These pictures were analyzed by Image J software for the liver area and fluorescence intensity. Compared with the control group,the liver area and liver fluorescence intensity of emodin group,rhein group,aloe emodin group,emodin-1-O-glucoside group,physcion-8-O-glucoside group,aloe emodin-8-O-glucoside group and positive control group (acetaminophen) decreased significantly,but those of chrysophanol group,physcion group,emodin-8-O-glucoside group,rhein-8-O-glucoside group,chrysophanol-1-O-glucoside group,chrysophano l-8-O-glucoside group,aloe emodin-3-hydroxy methyl glucoside group,resveratrol group,gallic acid group,catechin group,epicatechin group had no significant difference,except the tetrahydroxystilbene glucoside group,which was significantly higher. Thus it could be seen that emodin,rhein,aloe emodin,emodin-1-O-glucoside,physcion-8-O-glucoside,aloe emodin-8-O-glucoside had certain toxic effects on zebrafish larvae liver.The hepatotoxicity of polygoni multiflori radix may be mediated by anthraquinone compounds and its material basis is related to the 6 anthraquinone compounds mentioned above,which were mainly on the combined anthraquinones.

Key words: zebrafish, polygoni multiflori radix, hepatotoxicity, anthraquinone