Abstract: This study was aimed to investigate whether the ethanol extract of petroleum ether phase from Pumpkin Seeds had impactson the prostatic hyperplasia in mice.The results showed that 80% and 90% ethanol extract of petroleum ether phase from Pumpkin Seeds was the most sensitive part on the prostatic hyperplasia activity inhibition.Then three pure 1,4-cyclohexenyl ester monomer compounds were further separated from this part by ODS column:3-carboxylic acid-1,4-cyclohexadiene-2-undecanoate,3-carboxylic acid-1,4-cyclohexadiene-2-undecylenic acid methyl ester,Methyl 2-undecenoate-3-undecanoate-cyclohexyl-1,4-dienyl ester.These three compounds are anti-prostate active site separation,identification of esters from Pumpkin Seeds.Whether they have anti-prostatic hyperplasia active ingredients,but also need further study.

Key words: pumpkin seeds, anti-prostatic hyperplasia, isolation, structural identification