Abstract: In order to establish purification method of shikimic acid in water extract from pine leaves of Cedrus deodara.The alcohol precipitation,macroporous adsorption resin column chromatography and macroporous adsorption resin column chromatography binding different column chromatography method were explored with the content of shikimic acid as indexes.The purification process flow of shikimic acid was given as follows:water extraction-alcohol precipitation-column chromatography-recrystallization.Among which the purity of shikimic acid can be increased from 14.87% to 20.08% by alcohol precipitation method and increased from 20.08% to 35.73% by macroporous adsorption resin column chromatography method which the recovery of shikimic acid was higher than 95%,nearly white solid shikimic acid with the purity of 80.05% can be obtained by macroporous adsorption resin column chromatography binding silica gel column chromatography method and the recovery of shikimic acid was up to 60.04%,shikimic acid white power with the purity of 96.54% was obtained by recrystallization withdichloromethane and methanol.The results showed the purification method is simple,rapid,repeated well and suitable for industrial production of shikimic acid from pine leaves of C.deodara.

Key words: pine leaves of Cedrus deodara, shikimic acid, purification, ethanol precipitation, column chromatography