Abstract: To investigate the effect of combined prescription and separate prescription of Huayu Tongluo traditional Chinese medicine upon 24 h urine protein quantitation,blood lipid,platelet activation index and the expression of ZO-1 in podocytes of rats with diabetic nephropathy.Totally 60 male sprague-dawley rats were randomly selected as the normal control group (C group,n=10),the other 50 rats were injected with 1% streptozotocin (35 mg/kg) after feeding high fat and high glucose diet for one month.The diabetic model rats (blood glucose≥16.7 mmol/L) were randomly divided into the model group (M group),Huayu Tongluo traditional Chinese medicine group (Q group),Tongluo group (T group),Huayu group (H group).According to the body surface area ratio,the dosage of rats in each group was calculated.The rats in C group and M group were fed with the equal volume of drinking water,once daily for 16 weeks.24 h urine protein quantitation (24 h UTP),blood lipid and platelet activation index of the rats were detected at the end of the 16th week.The protein and mRNA expression of ZO-1 in kidney tissue were observed by Western blotting and Real-time PCR methods.Compared with C group,blood sugar,24 h UTP were significantly increased in other groups (P<0.01).Blood lipid and platelet activation index were significantly changed (P<0.05，P<0.01).Compared with M group,24 h UTP in each group of Chinese medicine was significantly reduced (P<0.01).Blood lipid and platelet activation index were improved significantly (P<0.05,P<0.01).Compared with C group,the expression of ZO-1 reduced in other groups (P<0.01).Compared with M group,the expression of ZO-1 were significantly higher in each group of Chinese medicine (P<0.01).Compared with Q group,the expression of ZO-1 was significantly reduced in H,T group (P<0.05,P<0.01).The results indicated that Huayu Tongluo Chinese medicine can reduce 24 h UTP in DN rats.The mechanism may be related to the regulation of lipid metabolism disorder,the improvement of blood clotting state and the up-regulation of the expression of ZO-1 and Huayu Chinese medicine and Tongluo Chinese medicine have synergistic effect to provide experimental basis for the compatibility of clinical Chinese medicine.

Key words: diabetic nephropathy, Huayu Tongluo traditional Chinese medicine, urine protein, blood lipid, ZO-1