Abstract: The aim of this study is to observe the effects of gastrodin (Gas) and new gastrodin derivative (Gas-D) on neurological deficit and cerebral infarct volume after cerebral ischemia/reperfusion in rats and the safety evaluation.Cerebral ischemia/reperfusion rat model was induced by the modified middle cerebral artery occlusion method.The rats were randomly divided into 6 groups:Sham group;Model group;Gas 50 (Gas 50 mg/kg) group (one time);Gas 50 (Gas 50 mg/kg) group (two times);Gas-D 50 (Gas-D 50 mg/kg) group (one time);Gas-D 50 (Gas-D 50 mg/kg) group (two times).The role of Gas-D on brain injury was evaluated by the neurological deficit scores and TTC staining.ICR mice were randomly divided into 2 groups,6 males and females per group.They were given intraperitoneally 2.0 g/kg Gas and Gas-D.Activity,diet consumption,mental condition,body weight and deaths were recorded at 1,7,14 d after treatment.The results showed that Gas-D 50 group (two times) improved neurological function and reduced the infarct volume.In acute toxicity experiments,there are no dead mice in Gas-D group at dose of 2.0 g/kg.The findings showed that Gas-D had neuroprotective effects and low toxicity.

Key words: cerebral ischemia, reperfusion injury, gastrodin, gastrodin derivative