Abstract: In order to investigate bioactive metabolites from endophytic fungi,a fungal GZWMJZ-068 was isolated and identified as Penicillium mallochii from the leaf of Eucommia ulmoides in Guiyang Guizhou,China.The extract of the fermentation was purified by column chromatography over silica gel,Sephadex LH-20 and semi-preparative HPLC.The structures were identified by means of NMR，MS and ORD.As a result,15 compounds,isochromophilones IV(1)、epi-isochromophilone III(2)、(2R,3S)-1-(4-Hydroxyphenyl)- butane-2,3-diol(3)、(2S,3S)-1-(4-Hydroxyphenyl)butane-2,3-diol(4)、6-［1-Hydroxy-(1S)-pentyl］-4-methoxy-(6S)-2H,5H-2-pyranone(5)、6-(1-hydroxypentyl)-4-methoxy-pyran-2-one(6)、quinolactacins Al(7a)、quinolactacins A2(7b)、dihydrocitrinone(8)、dihydrocitrinin(9)、protocatechuic acid(10)、pyrocatechuic acid(11)、5-Methyluracil(12)、uracil(13)、citrinal A(14).The antibacterial activities of 1-14 against 9 pathogenic microorganisms were tested by two-fold dilution method.Compounds 1 and 2 were active against Staphylococcus aureus and Candida glabrata with the minimum inhibitory concentration(MIC)values from 16 μg/mL to 32 μg/mL,respectively.

Key words: Eucommia ulmoides, endophytic fungi, secondary metabolites, antibacterial activities