Abstract: To find out the best extraction conditions of Carotenoids from Lily Pollen.With the yield of carotenoid extract as the response value,response surface methodology design coupled with the single factor experiments involving there factors,extraction temperature,extraction time and liquid-material ratio,was applied to set up a quadratic polynomial regression equation for Carotenoids extraction.An extraction regression equation was established through orthogonal experiments and the optimal extraction processing parameters were petroleum ether as extraction solvent,extraction temperature of 44 ℃,extraction time of 55 min and liquid-material ratio of 1∶34.Under the optimal extraction conditions,the extraction rate of carotenoids was 6.271 mg/g.

Key words: lily pollen, carotenoids, yield, organic solvent method, response surface analysis