NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2018, Vol. 30 ›› Issue (增刊2): 185-189.

• Article • Previous Articles     Next Articles

Construction of Ebola Pseudovirus and Screening of Antiviral Activity of Traditional Chinese Medicine

HOU Xue-wen,TIAN Jing-zhen,CUI Qing-hua*   

  1. Shandong University of Tranditional Chinese Medicine,Jinan 250355,China
  • Online:2019-01-18 Published:2019-01-18

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Abstract: In this study,Ebola,H5N1 and Lassa Pseudoviruses were constructed from 293T cells transfected with virus plasmids and HIV plasmids by cell-level recombinant virus technology.Luciferase fluorescence substrate was used to detect infection efficacy.After screening,32 drugs were screened for Ebola virus.After eliminating the toxic factors through toxicity screening,26 anti-Ebola herbal medicines,such as earthworm,50% ethanol extract and 75% ethanol cold extract,were obtained.The next step is to further extract and purify Ebola,and to find the effective components or components of Ebola virus in vitro.

Key words: pseudovirus, transfection, Ebola virus, H5N1 virus, Lassa virus

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