Abstract: In this paper,emulsin could convert ginsenoside Rg₁ to the single pruduct of rare ginsenoside Rh₁.The pathway of the transformation was Rg₁ → Rh₁.The reaction did not take place at room temperature for 5 days,while the reaction happened at 50 ℃ for 5 days and Rh₁ was obtained.And emulsin could transform ginsenoside Rb₁ to the mixture of Rd and rare ginsenoside C-K.The transformation pathway of ginsenoside Rb₁ to C-K was Rb₁→Rd→C-K.Rd was obtained after treated with emulsin at room temperature for 5 days,Rd and C-K was obtained while treated with emulsin at 50 ℃ for 5 days.The products were separated and purified by silica gel column chromatography.The structures of the hydrolyzates were identified with ¹³C NMR,1H NMR and MS spectra.The reaction conditions of emulsin transform ginsenoside Rg₁ and Rb₁ are mild and the byproducts are rare.

Key words: ginsenoside Rg₁, ginsenoside Rb₁, rare ginsenoside Rh₁, rare ginsenoside Rd, rare ginsenoside C-K, emulsin