NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2019, Vol. 31 ›› Issue (1): 122-128.doi: 10.16333/j.1001-6880.2019.1.019

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Phytochemical,antioxidant and antibacterial activities of Achillea millefolium L.

LI Hong-liang1,2,3,LI Jun1,2,ZHAO Hai-qing1,2,Ha·AISA1,2*   

  1. 1Key Laboratory of Plant Resources and Chemistry in Arid Zone,Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Sciences; 2State Key Laboratory Breeding Base of Xinjiang Indigenous Medicinal Plant Resource Utilization Co-founded by Xinjiang Provinceand MOST,Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Urumqi 830011,China; 3University of Chinese Academy of Sciences,Beijing 100049,China
  • Online:2019-01-28 Published:2019-01-28

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Abstract: For the aim of reveal the bioactivities and corresponding chemical constituents of Achillea millefolium L.distributed in Xinjiang,China,investigations of isolation,structure elucidation,and bioactivity on A.millefolium collected from Hami region of Xinjiang were carried out.Twenty compounds were isolated from the whole plant of A.millefolium L by various techniques including column chromatography and semi-preparative HPLC.Their structures were identified based on spectroscopic data analysis such as NMR and MASS,together with comparison with previous data,to be centaireidin (1),chrysosplenetin (2),quercetin (3),apigenin (4),apigenin-7-O-β-D-glucoside (5),(-)-sesamin (6),ethoxy-ferulate (7),ethyl caffeate (8),p-couaric acid (9),p-hydroxy benzenepropanoic acid (10),umbelliferone (11),7-methoxy coumarin (12),vanillic acid (13),4-hydroxybenzoic acid (14),dihydroactinidiolide (15),paeoveitol B (16),2-hydroxyl-2-［(E)-1α,2β,3-trihydroxyl-3-nonaene-5,7-diyne］-4H-pyran (17),indole-3-aldehyde (18),β-sitosterol (19),and ergosterol peroxide (20).Among them,compound 20 was isolated from this plant for the first time,and ten compounds (6,8~10,12~13 and 15~18) were firstly discovered from Achillea genus.In addition,antioxidant and antimicrobial activities of the ethanol extract,ethyl acetate fraction,and compounds 1~5,8~9,13~14 and 16 were also evaluated.Compounds 3 (IC50=9.9±0.94 μM) and 8 (IC50=21.29±1.65 μM) showed strong DPPH free radical scavenging capacity.Compounds 9,14 and 16 were found to inhibit the growth of Candida albicans,Escherichia coli and Staphylococcus aureus.This was the first report on the chemical constituent and bioactivity of A.millefolium growing in Xinjiang.Phenolic compounds including flavonoid and phenylpropanoid were found to be the major components in this plant.Moreover,the discovery of antioxidant and anti-microbial activities of compounds in A.millefolium supported the medicinal values of this plant.

Key words: Achillea millefolium L., flavonoid, phenylpropanoid, antioxidant, antimicrobial

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