Abstract: To observe the effect of Huayu Jiedu decoction on macrophages infiltration in obstructive nephropathy rats,48 male Wistar rats were randomly divided into sham group,UUO group,EPL group and ZY group (n=12).Except the rats in sham group,the rats in the other groups were prepared unilateral ureteral obstruction (UUO) for renal interstitial fibrosis model.The rats were treated with 14 g/kg/d decoction of Chinese medicine in ZY group orally,and eplerenone at 100 mg/kg/d added to diet in EPL group.The kidneys were harvested to observe the histopathological changes at 10th day after surgery.Macrophage infiltration marked with F4/80 was examined by immunohistochemistry.The Serum and glucocorticoid induced kinase-1(SGK-1),Monocyte chemotactic protein-1(MCP-1),interleukin-1(IL-1) and tumor necrosis factor-α(TNF-α) were detected with immunohistochemistry and western blot.Renal pathology showed that renal tubular dilatation and epithelial cell shedding in UUO rats significantly.The infiltrated macrophage and accumulated extracellular matrix (ECM) also increased in interstitium of UUO rats markedly.The macrophages infiltration and ECM accumulation were attenuated by treated with Chinese herb decoction and eplereneone.Expressions of SGK-1,MCP-1,IL-1 and TNF-α were up-regulated in the UUO rats and were suppressed by Chinese herb decoction.Chinese herb decoction could inhibit the inflammatory injury via reducing macrophage infiltration in UUO rats.

Key words: Huayu Jiedu decoction, aldosterone, inflammatory injury of kidney, macrophage, UUO