Abstract: The aim of this study was to investigate the expression of function gene in the seedling of Paris polyphylla var.yunnanensis inoculated by different foreign arbuscular mycorrhizal (AM) fungi species.With sterilized soil as the growth of the substrate,the fresh seeds of P.polyphylla var. yunnanensis were co-cultured with 28 AM fungi species under the condition of pot culture at room temperature.The differential expression of four functional genes including Squalene epoxidase(SE),Symbiosisreceptor-like kinase (SYMRK),Doesn’t making fections 1(DMI1),Calcium/calmodulin-dependent protein kinase(CCaMK),were detected by real-time fluorescence quantitative PCR.The results showed that the 28 strains of AM strains could affect the expression of four functional genes SE,SYMRK,DMI1 and CCaMK in different extent.Ambispora leptoticha (Ale) and Archaeospora trappei (Atr) can significantly increase the expression of the four functional genes.Paraglomus occultum (Po) and Scutellospora pellucida (Spe) can increase the expression of SE,SYMRK;Acaulospora scrobiculata (Asc),Racocetra fulgida (Rfu),Entrophospora colombiana (Ec),Rhizophagus clarus (Rcl) and Rhizophagus intraradices (Rin) can increase the expression of DMI1.In conclusion,the expression of four functional genes was significantly increased by Ale and Atr in 28 strains of AM fungi.There was obvious influence that the seed germination and seedling chemical constituents of Paris polyphylla var.yunnanensis inoculated by different foreign arbuscular mycorrhizal fungi species in our previous study.It is presumed that these two strains (Ale and Atr) are expected to serve as the ideal strains for the mycorrhizal seedlings cultivation of Paris polyphylla var.yunnanensis .Artificial inoculation of AM fungi can open up a new way for the protection and improvement of Paris polyphylla var.yunnanensis.

Key words: Paris polyphylla var. yunnanensis, arbuscular mycorrhizal fungi, gene expression