Abstract: Protein hydrolysates,a mixture of amino acids and peptides,were prepared through the hydrolysis of proteins by enzyme,acid,alkali and fermentation methods.Protein hydrolysates had been widely used in the field of biotechnology due to that the hydrolyzatescould provide a variety of nutrients,adherent factors and growth factor analogs for cell growth.This review summarized the preparation of protein hydrolysates,the application of hydrolysates in the field of biotechnology,and the effects of hydrolysates on cell culture,proliferation and metabolism in order to provide basis for the further study and application of protein hydrolysates.

Key words: protein hydrolysates, preparation process, application in biotechnology fields, cell culture