NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2019, Vol. 31 ›› Issue (3): 506-511. doi: 10.16333/j.1001-6880.2019.3.022

Special Issue: No.3

• Article • Previous Articles     Next Articles

The improvement and the mechanism of impaired islets in diabetes mellitus of Edgeworthia gardneri（Wall.）Meissn

WANG Ya-xin1,ZHANG Zhi-wen1,REN Yi-lin2,3,LIU Min4,SHI Jin-song1,XU Zheng-hong2,3,XU Hong-yu2,3*   

  1. 1School of Pharmaceutical Science,Jiangnan University; 2Bioengineering Institute,Key Laboratory of Industrial Microbiology of Ministry of Education,Jiangnan University; 3National Engineering Laboratory for Cereal Fermention Technology,Jiangnan University,Wuxi 214122,China; 4National United Engineering Research Center for Tibetan Plateau Microbiology,Lasa 850000,China
  • Online:2019-04-02 Published:2019-04-02

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Abstract: The purpose of this study was to evaluate the hypoglycemic effect and protective effect of hexane extract (EGH) of Edgeworthia gardneri (Wall.) Meissn and to explore its mechanism through islet cell damage model.The model of type 2 diabetic mice induced by streptozotocin (STZ) combined with high fat diet was used to evaluate the effect of EGH on reducing the glucose and protecting the islet,by testing the fasting blood glucose,oral glucose tolerance,HbAlc and insulin secretion.On the model of RIN-m5F cell injury induced by palmitic acid combined with glucose,the level of ROS,caspase-3 gene,AKT,FOXO1 and JNK protein were detected to explore the mechanism of EGH to improve islet injury.The results showed that EGH could reduce the amount of ROS generation,reduce the transcription level of caspase-3 gene,activate AKT,inhibit FOXO1and inhibit the activation of JNK,thus improving the damage of β-cell.

Key words: diabetes, impaired islets, Edgeworthia gardneri (Wall.) Meissn, hypoglycemic effect, signaling pathway

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