Abstract: The purpose of this experiment was to investigate whether or not the industrial medicinal residue of Panax notoginseng contains dencichine,how to extract,isolate and purify the dencichine,and to investigate the hemostatic pharmacological activity of the dencichine.The industrial Panax notoginseng medicinal residue from which the total saponins of Panax notoginseng has been extracted is used as a raw material,using water extraction and alcohol precipitation method,filtering the precipitate to obtain a filtrate，the ethanol solution in the filtrate is recovered to obtain a crude extract,and the crude extract is separated by a 001 × 7 cationic resin column,the thin layer identification method was used to detect whether the eluate contained dencichine,and the eluate with the same spots as the reference substance of the dencichine was collected,crystallized by acetone,and lyophilized to obtain the sample of the dencichine.Determination of the content of dencichine in the samples of dencichine by high performance liquid chromatography and the clotting time of the samples of dencichine by slide method.The HPLC method was established for the determination of notoginseng content.The accuracy,precision and stability of the method were good.The content of dencichine in the samples of dencichine was 59.03%.The intraperitoneal injection of different doses of dencichine in the mice compared with the positive group can significantly shorten the clotting time.The established high performance liquid chromatography method can be used to determine the content of the test sample of dencichine;the test sample of dencichine can significantly shorten the clotting time.The content of dencichine in the sample of dencichine was reversed to 0.72% in the industrial Panax notoginseng medicinal residue.The low-cost 001 × 7 cation exchange resin column was used to separate and purify the notoginseng.This method is simple and easy,and the cost is low.Suitable for large industrial production.In summary,the extraction and purification of dencichine from industrial Panax notoginseng medical residue has a good comprehensive utilization prospect.

Key words: Panax notoginseng, dencichine, 001×7 cation exchange resin, HPLC, slide method