Abstract: To optimize the extraction condition for the polysaccharide from Pteridium aquilinum and the pharmacological activities of polysaccharide were investigated in the study.The method of ultrasonic-assisted extraction was used,and on the basis of single factor test,the solid-liquid ratio,extraction times,extraction time and ultrasonic power were selected for the four-factor to target the influence as the yield of polysaccharide.Design expect 10.0 software was employed to analyze the test data and the response surface method (RSM) was applied to optimize the extraction conditions.Additionally the pharmacological activities of on tow radicals such as lymphocyte and colon cancer (HCT-8) cells proliferation were subsequently analyzed.The results suggested that optimum extraction consisted of a ration of the powder to reagent of 36.2∶1 and extraction times of 4 and extraction time of 43.1 min each time with the ultrasonic powder of 240 W.The extraction yield of polysaccharide from P.aquilinum reached to 8.60 %.The sequence of factors that worked on the extraction rate of polysaccharide was as follows:solid-liquid ratio > extraction times > extraction time > ultrasonic power.Moreover,the pharmacological activities experiment showed that polysaccharide extracted from P.aquilinum exhibited stronger activities to enhance hematopoiesis and inhibiting the HCT-8 cells growth.

Key words: Pteridium aquilinum, response surface methodology, polysaccharide, immune enhancement, anti-tumor