Abstract: To investigate the differences of chemical composition and cytotoxic activity between two medicinal fungi,Shiraia bambusicola and Hypocrella bambusae,high performance liquid chromatography analysis and chromatographic separations were conducted.Six compounds were isolated from both fungi and characterized as hypocrellin A (1),hypocrellin B (2),hypocrellin C (3),3,6,8-trihydroxy-1-methylanthone (7),3,8-dihydroxy-6-methoxy-1-methylanthone (8) and ergosterol peroxide (9),respectively.In addition,11,11′-dideoxyverticillin (5),ergosta-7,22E-dien-3β,5α,6β-triol (10) and ergosta-7,22E-diene-2β,3α,9α-triol (11) were also obtained from S. bambusicola,while hypocrellin D (4),(+)-griseofulvin (6),compounds 7 and 8 were reported from H. bambusae for the first time.Compound 5 showed potent inhibitory activity against human lung adenocarcinoma cell (NCI-H1975),human carcinoma (HepG2) and human breast cancer cells (MCF-7).Compound 1 also showed significant inhibitory activity against the three cell lines.In contrast,the cytotoxic activity of compound 6 was relatively weak.

Key words: Shiraia bambusicola, Hypocrella bambusae, chemical constituents, cytotoxic activity