Abstract: This paper aims to explore the differences in the metabolism of ginsenoside Rg1,Re,Rb1 and Rd in the Panax notoginseng decoction of human or rat gut microflora,and the discovery of the metabolites 20(S)-protopanaxadiol type (PPD) and 20(S)-protopanaxatriol type (PPT).The content of 4 kinds of saponins in the incubating solution and the content of PPD and PPT of the metabolites were measured by UPLC method after the Panax notoginseng water decoction was incubated with the gut microflora of human or rat respectively under the anaerobic condition.The results showed that the contents of ginsenoside were Rg1 9.450 0 mg/g,Re 1.887 2 mg/g,Rb1 8.581 6 mg/g,Rd 1.945 6 mg/g in the Panax notoginseng respectively.After co-culture with human gut microflora,the content of 4 kinds of ginsenosides in Panax notoginseng was significantly reduced.It was important that the contents of metabolites PPD and PPT were detected in the culture medium,the content was 0.213 6mg/g and 0.034 4mg/g respectively,and the Rb1 and Rd content in Panax notoginseng was slight decrease after co-culture with the gut microflora in rats.While the content of Rg1 and Re was not changed significantly,but a small amount of PPT (0.018 4 mg/g) was formed.It could be concluded that under the conditions of in vitro,ginsenosides in Panax notoginseng decoction can be degraded by human gut microflora to produce metabolites PPD and PPT,but only PPT in rats,there are significant different between species.

Key words: gut microflora, Panax notoginseng, ginsenoside Rg1, ginsenoside Rb1, ginsenoside Re, ginsenoside Rb1