Abstract: In order to develop a new type of natural tobacco spice,Akebia trifoliata was used as raw material to separate the flavoring substances,and it is applied to tobacco processing.The effects of different column fillers and ethanol concentration on the aromatic addition of cigarette were investigated.The separation and purification conditions of the Akebia trifoliata extracts applied to the cigarette flavoring were determined,and the chemical basis of the best preparation was studied Six compounds were isolated and purified from the extract by means of high-performance preparation chromatography and gel column,and the chemical structure was determined by combining physical and chemical properties using ESI-MS,¹H NMR,¹³C NMR and other spectral methods.They are:Isovanillin acid (1),neochlorogenic acid methyl ester (2),3,5-dicoffee methyl acyl quinone (3),osmanthuside E (4),xyphenyl glycoside B(5) and 2-(4-hydroxyphenyl)ethyl-(6-O-feruloyl)-β-D-glucopyra-noside (6).The results showed that the use of AB-8 as a filler and 50% ethanol as the eluent to separate and refine the sample was the most effective,which could significantly enhance the fragrance,enhance the sweetness,improve the stimulation,soft smoke.

Key words: Akebia trifoliata, cigarette, flavoring, chemical components