Abstract: To establish a UPLC method for simultaneous determination of dictamnine,obacunone and fraxinellone in Dictamni Cortex.The analysis of methanol extract of Dictamni Cortex was performed on a 35 ℃ Waters BEH C₁₈ column (2.1 mm×50 mm,1.7 μm),with the mobile phase comprising of acetonitrile-water flowing at 0.4 mL/min in a gradient elution manner,and the detection wavelength was set at 236 and 213 nm.Dictamnine,obacunone and fraxinellone showed good linear relationships within the ranges of 0.501 6-20.06 μg/mL (r=0.999 5),4.496-179.8 μg/mL (r=0.999 6),4.003-160.1 μg/mL (r=0.999 7),whose average recoveries were 98.96 %,99.63%,99.30% with the RSDs of 1.25%,1.71%,1.34%,respectively.This simple,accurate and reproducible method can be used for the quality control of Dictamni Cortex.

Key words: Dictamni Cortex, dictamnine, obacunone, fraxinellone, UPLC