Abstract: Observe the therapeutic effect of Nobiletin on rats with diabetic nephropathy.120 SD rats were divided into the normal group (20 rats) and diabetic nephropathy model group (100).After the model was successfully constructed,50 rats were selected and divided into the model group,low-dose,medium-dose and high-dose Nobiletin groups and the benazepril group(n=10).After treatment for 6 weeks, they were sacrificed,their urine was collected to detect the 24 h urine volume and 24 h-urine protein,and their blood to observe the blood glucose,insulin,blood lipids,renal function indicators and inflammation indexs,at last the renal pathology and the expression of apoptosis-related proteins Bcl-2,Bax and Caspase-3 in kidney tissues were detected.The results of HE staining could be seen the glomerular enlargement,partial mesangial hyperplasia and interstitial fibrosis could be observed in the model group.Compared with the model group,the slices in the benazepril group and nobiletin group were improved.Compared with the model group,the benapril group and the nobiletin low,medium and high dose group for the UCr,24h proteinuria,BUN,Scr,blood glucose,TG,TC,IL-1,IL-6 and TNF-α were significantly decreased (P<0.05),and insulin was significantly increased (P<0.05).Compared with the model group,in the benazepril group and nobiletin group the Bax and Caspase-3 were significantly decreased (P<0.05) and Bcl-2 was significantly increased (P<0.05).Above results suggested the nobiletin has obvious protective effects on renal function in rats with diabetic kidney damage.〖JP〗

Key words: nobiletin, diabetic nephropathy, rat, apoptosis-related protein