Abstract: In order to study the sedative and hypnotic effects of inhalation administration of compound anshen essential oil,this experiment used animal autonomic activity test and pentobarbital sodium-induced sleep latency and sleep time experiments.GC-MS analysis of the volatile chemical constituents of the compound anshen essential oil and the main chemical components into the brain,based on network pharmacology prediction and screening for the treatment of insomnia-related target proteins and active ingredients.It aims to provide a theoretical basis for the treatment of compound anshen oil and improve insomnia.The study found that the compound anshen essential oil has sedative and hypnotic effect,which can significantly reduce animal autonomic activity,shorten sleep latency and prolong sleep time.The main chemical components of compound anshen essential oil and the main chemical components into the brain contain many kinds of chemical components which have the sedative and hypnotic effect on the nerve center,anti-anxiety effect and anti-depression effect.Based on network pharmacology prediction and screening,25 insomnia-related active ingredients acted on 39 therapeutic insomnia-related targets,reflecting the characteristics of multiple components and multiple targets of compound anshen essential oils to exert sedative and hypnotic effects.

Key words: compound anshen essential oil, aromatherapy, inhalation administration, sedative and hypnotic, inhaled into the brain, network pharmacology