NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2019, Vol. 31 ›› Issue (10): 1731-1737. doi: 10.16333/j.1001-6880.2019.10.009

• Article • Previous Articles     Next Articles

Determination of five active components in Uncaria rhynchophylla leaves

HUANG Shu-ting1,HUANG Yan-jun2,LIANG Ying-xin1,LAI Zhi-ming3,WEN Sheng-xiang4,ZENG Chang-qing1*   

  1. 1School of Traditional Chinese Medicine & Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of TCM,Guangdong Pharmaceutical University,Guangzhou 510006,China; 2Zhongshan Zhongzhi Pharmaceutical Group Co.,Ltd.Zhongshan 528437,China;3 Guangdong Nanling Pharmaceutical Co.,Ltd.Meizhou 514600,China;4Uncaria Research Institute,Jianhe County Science and Technology Bureau,Guizhou Province,Jianhe 556400,China

  • Online:2019-10-28 Published:2019-11-11
  • Supported by:

    贵州省剑河县中药材产业科技合作专项(剑科合字(2014)4号)

Abstract: The determination method of five active components in the leaves of Uncaria rhynchophylla(Miq.) Miq.ex Havil was established to make up for the deficiency of the quality control method of U.rhynchophylla leaves.In this paper,the antioxidant activity sites of U.rhynchophyllaleaves were traced,and the antioxidant activity of U. rhynchophylla leaves was mainly related to flavonoids and polyphenols.HPLC-PDA method was used to determine the content of five active ingredients such as chlorogenic acid,epicatechin,rutin,hyperoside and vincoside lactam in U. rhynchophylla leaves by gradient elution mode with acetonitrile-0.1% phosphoric acid.The five components were separated well in the chromatographic conditions.The RSDs of the precision,stability and repeatability test were all <3.0%,and the average recoveries were between 96.73%-103.50%.The cluster analysis of 12 batches of samples at different regions showed that the content of the five components was related to geographical distribution of the regions.The established HPLC method is simple in operation,good in accuracy and high in precision,and provides a basis for comprehensive development and utilization of U. rhynchophylla leaves.

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