Abstract: A HPLCMS method was applied to analyze the chemical constituents of the nucleosides in Cordyceps cicadae.A HPLC method for simultaneous determination of 6 components (adenine,uridine,inosine,guanosine,adenosine,and N6(2hydroxyethyl) adenosine) was established.Thirteen nucleosides were identified.The adenine,uridine,inosine,guanosine,adenosine and N6(2hydroxyethyl) adenosine showed good linear relationship（r＞0.999 1）in the range of 2.3418.67,47738.13,3.4827.87,1.139.07,4.7638.04,2.8422.76 μg/mL,respectively.The average recoveries rate（n=6）were 97.32%~102.37%,and the precision,repeatability and stability were good.It can be used to analyze the contents of main nucleosides in C. cicadae. There were some differences in the contents of nucleosides in the C. cicadae from different habitats.The total contents of 6 nucleoside were relatively higher in the C. cicadae from Dabieshan and Jurong.Conversely,the contents were relatively lower in those from Tianmushan,Xuancheng and Sanming.Compared the different parts of C. cicadae,the contents of nucleosides including adenine,uridine,adenosine,guanosine and inosine in synnemata were higher than in sclerotium.While,the content of N6(2hydroxyethyl) adenosine in sclerotium was higher than in synnemata.The results can provide a reference for the quality control and further development and utilization of C. cicadae.

Key words: Cordyceps cicadae, high performance liquid chromatographymass spectrometry, different habitats, different parts, nucleoside, content determination