Abstract: This paper reported the chemical constituents and their activity of scavenging DPPH free radicals in the flower of Matricaria chamomille.Eleven compounds were isolated from Matricaria chamomille by chromatography of silica gel,ODS,Sephadex LH20 and preHPLC,and their structures were elucidated by chemical properties and spectroscopic analyses.Their structures were elucidated as kaemperol-3-O-β-D-glucopyranoside (1),luteolin -3′-O-β-D-glucopyranoside (2),6-hydroxyluteolin-7-O-β-D-glucoside (3),isoquercitrin (4),5-hydroxy-4′,7-dimethoxy-6,8-dimethylflavone (5),quercetin-3′ -O-β-D-glucoside (6),3-hydroxy benzenemethanol (7),1-(4-hydroxyphenyl) ethane-1,2-diol (8),2-(3- methoxy-4-hydroxyphenyl)propane-1,3-diol (9),2-(4-hydroxyphenyl)ethanol (10),p-Hydroxybenzoic acid (11).Among which compounds 13,5,7-11 obtained from this plant for the first time.After DPPH radical scavenging assay,compounds 13,5 and 6 exhibited signficant activity.

Key words: Matricaria chamomilla, chemical composition, free radical scavenging activity, DPPH