Abstract: Astragalus membranaceus from different producing areas in Qinghai province,and the correlation between soil and growth index and the content of effective components was studied,so as to provide references for the comprehensive quality evaluation of Astragalus membranaceus from different producing areas in Qinghai province and scientific basis for the standardized cultivation in producing areas.Agilent HC-C₁₈ (250 × 4.6 mm,5μm) column was used.The mobile phase was acetonitrile and 0.01% phosphoric acid water with gradient elution.The flow rate was 1 mL/min,the column temperature was 30 ℃,and the detection wavelength was 260 nm.The growth index of Huangzhong County is better,but that of Ledu County is worse.Taking yield as index,Huangzhong County > Huzhu County > Minhe County > Huangyuan County > Datong County > Ledu County.Astragalus mongolica from different habitats has different contents of anthocyanin and isoflavone of stamen.Among the six counties,the content of mangiferin was Datong County > Ledu County > Minhe County > Huzhu County > Huangyuan County > Huangzhong County;the content of stamen isoflavone was Huzhu County > Datong County > Ledu County > Huangzhong County > Minhe County > Huangyuan County.Taking the content of Ononoside as the harvesting index,Datong County as the suitable harvesting area,mulberry isoflavone was taken as harvesting index and Huzhu County as suitable producing area.Ledu County as the best harvesting area by synthesizing two content indexes and cluster analysis.However,Huangyuan County is the best harvesting area with appearance and yield as harvesting indicators.The content of Ononoside in 19 producing areas of Qinghai Province is not less than 0.294 mg/g.It is suggested that the content of ononoside in Astragalus mongolica should not be less than 0.02% as one of the quality evaluation indexes for improving the quality of Astragalus mongolica in Qinghai Province.

Key words: Different habitats, Astragalus mongolica, Ononoside, Mulberry isoflavone