Abstract: The phylogenetic analysis of Streptomyces strain FSG-003 was carried out by PCR amplification of 16S rDNA,and its physiological and biochemical characteristics were identified.Based on the published sequence of the  Streptomyces  16S rDNA gene,we designed amplification primers.A phylogenetic tree was constructed based on the amplified 16S rDNA sequence of FSG-003 for analysis of the genetic relationship between  Streptomyces  sp. FSG-003 and Streptomyces cyanus. In addition,the physiological and biochemical characterization showed that strain FSG-003 and  Streptomyces sphaeroides were close.Phylogenetic analysis using MEGA 7.0 by 16S rDNA sequencing of strain FSG-003 and other Streptomyces showed that FSG-003 and  Streptomyces  sp. strain SN0280 were in the same branch.These results demonstrated that FSG-003 was similar to SN0280 both in genetic composition and function.

Key words: Streptomyces, PCR-SSCP, 16S rDNA, phylogenetic analysis