Abstract: To apply affinity ultrafiltration coupled with liquid chromatography mass spectrometry (UF-HPLC-MS) and molecular docking to screen α-glucosidase inhibitor from Actinidia chinensis Planch roots.4-Nitrobenzene-α-D-glucopyranoside (PNPG) was used as a substrate and acarbose as a positive control drug to evaluate the α-glucosidase inhibitory activity of Actinidia chinensis Planch roots.The IC₅₀ of Actinidia chinensis Planch roots was at 1.55 μg/mL,stronger than acarbose whose IC₅₀ was at 75.97 μg/mL.Furthering extracted with petroleum ether,ethyl acetate and n-butanol.The IC₅₀ of the three sites inhibiting α-glucosidase were 17.93,8.14,and 3.12 μg/mL.The results indicated the obvious α-glucosidase inhibitory activity of Actinidia chinensis Planch roots,we adopted affinity ultrafiltration to rapidly screen and identify the α-glucosidase inhibitors from Actinidia chinensis Planch roots,in which way,7 compounds were identified according to their MS/MS spectra.Among them, β-sitosterol,isoscopoletin and chlorogenic acid were analyzed by means of LC-MS coupling technique.The results demonstrated the effectiveness of the method and further demonstrated its binding activity by molecular docking.

Key words: roots of Actinidia chinensis Planch;affinity ultrafiltration;liquid chromatography-mass spectrometry, α-glucosidase;molecular docking