NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2020, Vol. 32 ›› Issue (1): 11-17.doi: 10.16333/j.1001-6880.2020.1.002

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Characteristics research on dynamic changes of secondary metabolites in Bletilla striata for different harvest time combined with chemical pattern recognition

QIN Ya-dong1,2,WANG Rong-bin1,2,FANG Feng-man2 *,LI Lin-hua1   

  1. 1Department of pharmacy,Anhui college of Traditional Chinese Medicine;2College of life sciences,Anhui Normal University,Wuhu 241002,China
  • Online:2020-01-28 Published:2020-03-25

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Abstract: To analyze the characteristic secondary metabolites and its dynamic change rule of cultivated Bletilla striata collected from different harvest time.The chemical fingerprints of 24 Bletilla striata samples collected from different harvest time was established by high performance liquid chromatography (HPLC) and the content of gastrodin、militarine were determined.Principal components analysis (PCA) and partial least squares-discriminate analysis (PLS-DA) were carried out on the HPLC fingerprints,16 common peaks were marked in the HPLC fingerprint,furthermore,7 characteristic secondary metabolites were found and identified (glucosides:gastrodin;benzyl ester:blestroside,militarine,gymnoside Ⅴ;phenanthrenes:4-methoxyphenanthrene-2,7-diol,coelonin,benzylhydrophenanthrene 3) by HPLC-high resolution mass spectrometry (HPLC-HRMS).Results indicated that these 7 chemical compounds were the characteristic secondary metabolites and showed different dynamic changes with seasons.The results were demonstrated to be very useful for guiding harvest time and quality assessment of Bletilla striata.

Key words: Bletilla striata, harvest time, HPLC-HRMS, fingerprint, secondary products, quality assessment

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